Siro Simizu

Academic Background

1989年 千葉市立千葉高等学校　卒業
1989年 慶應義塾大学　理工学部　Ⅲ系　入学
1993年 慶應義塾大学　理工学部　応用化学科　卒業
1993年 慶應義塾大学大学院　理工学研究科　生体医工学専攻　修士課程　入学
1995年 慶應義塾大学大学院　理工学研究科　生体医工学専攻　修士課程　修了
1995年 慶應義塾大学大学院　理工学研究科　生体医工学専攻　博士課程　入学
1998年 慶應義塾大学大学院　理工学研究科　生体医工学専攻　博士課程　修了
1998年 博士（工学）号　取得

Work History

1998年 理化学研究所　抗生物質研究室　基礎科学特別研究員
2000年 理化学研究所　抗生物質研究室　研究員
2007年 独立行政法人理化学研究所　長田抗生物質研究室　専任研究員
2010年 慶應義塾大学　理工学部　応用化学科　専任講師
2012年 慶應義塾大学　理工学部　応用化学科　准教授
2017年 慶應義塾大学　理工学部　応用化学科　教授
現在に至る

Publication List

Original Articles

  1. Kawaguchi M, Aoki H, Kamo H, Miura K, Hiruta Y, Simizu S & Citterio D. Simplified capture, extraction and amplification of cellular DNA from water samples. Anal. Sci. 40, 501-510 (2024)
  2. Baydoun H, Kato Y, Kamo H, Hüsch A, Mizuta H, Kawahara R & Simizu S. DPY19L3 promotes proliferation and vasculogenic mimicry in human cancer cell lines by its C-mannosyltransferase activity. Oncol. Res. in press
  3. Kage A, Okuyama Y, Kato E, Matagawa T, Kawano S, Simizu S, Chida N, Okamura T & Sato T. Synthesis and evaluation of saponins with all-nitrogenated sugars. Synlett 35, 427-430 (2024)
  4. Nishida H, Sasaki T, Taga Y, Murasawa Y, Simizu S, Matsushita S, Isogai Z, Hattori S, Daa T, Nagamine N, Sekine A & Fujiwara S. Presence of microfibril associated glycoprotein 4 and type V collagen and the possible absence of fibrillin-1 in bead-like structures in elastofibroma. J. Dermatol. Sci. 112, 112-116 (2023)
  5. Kawahara R, Usami T, Arakawa S, Kamo H, Suzuki T, Komatsu R, Hara H, Niwa Y, Shimizu E, Dohmae N, Shimizu S & Simizu S. Biogenesis of fibrils requires C-mannosylation of PMEL. FEBS J. 290, 5373-5394 (2023)
  6. Watanabe R, Nakachi Y, Matsubara H, Ueda J, Ishii T, Ukai W, Hashimoto E, Kasai K, Simizu S, Kato T, Bundo M & Iwamoto K. Identification of epigenetically active L1 promoters in the human brain and their relationship with psychiatric disorders. Neurosci. Res. 195, 37-51 (2023)
  7. Nakajima M, Kawahara R & Simizu S. Cofilin promotes vasculogenic mimicry by regulating actin cytoskeleton in human breast cancer cells. FEBS Lett. 597, 1114-1124 (2023)
  8. Yoshimoto S, Suzuki T, Otani N, Takahashi D, Toshima K, Dohmae N & Simizu S. Destabilization of vitelline membrane outer layer protein 1 homolog (VMO1) by C-mannosylation. FEBS Open Bio 13, 490-499 (2023)
  9. Soda Y, Sugiyama Y, Sato S, Shibuya K, Saegusa J, Matagawa T, Kawano S, Yoritate M, Fukaya K, Urabe D, Oishi T, Mori K, Simizu S, Chida N & Sato T. Total synthesis and anti-inflammatory activity of stemoamide-type alkaloids including totally substituted butenolides and pyrroles. Synthesis 55, 617-636 (2023)
  10. Mizuta H, Takakusaki A, Suzuki T, Otake K, Dohmae N & Simizu S. C-mannosylation regulates stabilization of RAMP1 protein and RAMP1-mediated cell migration. FEBS J. 290, 196-208 (2023)
  11. Ohno O, Iwasaki A, Same K, Kudo C, Aida E, Sugiura K, Sumimoto S, Teruya T, Tashiro E, Simizu S, Matsuno K, Imoto M & Suenaga K. Isolation of caldorazole, a thiazole-containing polyketide with selective cytotoxicity under glucose-restricted conditions. Org. Lett. 24, 4547-4551 (2022)
  12. Kawano S, Matagawa T, Matsuda Y, Koyama T, Miura K, Nakata M, Saikawa Y & Simizu S. Biological evaluation of anti-inflammatory effect of africane-type sesquiterpenoids. Bioorg. Med. Chem. 68, 116857 (2022)
  13. Kamo H, Kawahara R & Simizu S. Tyrosinase suppresses vasculogenic mimicry in human melanoma cells. Oncol. Lett. 23, 169 (2022)
  14. Saegusa J, Osada Y, Miura K, Sasazawa Y, Ogura A, Takao K & Simizu S. Elucidation of structure-activity relationship of humulanolides and identification of humulanolide analog as a novel HSP90 inhibitor. Bioorg. Med. Chem. Lett. 60, 128589 (2022)
  15. Matsuda Y, Koyama T, Kawano S, Miura K, Simizu S, Saikawa Y & Nakata M. Scalable syntheses and biological evaluation of africane-type sesquiterpenoids. Chem. Biodivers. 19, e202100890 (2022)
  16. Kawahara R & Simizu S. ErbB4-mediated regulation of vasculogenic mimicry capability in breast cancer cells. Cancer Sci. 113, 950-959 (2022)
  17. Shimizu S, Kawahara R & Simizu S. Methionine aminopeptidase-2 is a pivotal regulator of vasculogenic mimicry. Oncol. Rep. 47, 31 (2022)
  18. Mori K, Sun H, Miura K & Simizu S. Involvement of DPY19L3 in myogenic differentiation of C2C12 myoblasts. Molecules 26, 5685 (2021)
  19. Mori K, Suzuki T, Miura K, Dohmae N & Simizu S. Involvement of LH3 and GLT25D1 for glucosyl-galactosyl-hydroxylation on non-collagen-like domain of FGL1. Biochem. Biophys. Res. Commun. 560, 93-98 (2021)
  20. Mizuta H, Okada K, Araki M, Adachi J, Takemoto A, Kutkowska J, Maruyama K, Yanagitani K, Oh-hara T, Watanabe K, Tamai K, Friboulet L, Katayama K, Ma B, Sasakura Y, Sagae Y, Kukimoto-Niino M, Shirouzu M, Takagi S, Simizu S, Nishio M, Okuno Y, Fujita N & Katayama R. Gilteritinib overcomes lorlatinib resistance in ALK-rearranged cancer. Nature Commun. 12, 1261 (2021)
  21. Miura K, Kawano S, Suto T, Sato T, Chida N & Simizu S. Identification of madangamine A as a novel lysosomotropic agent to inhibit autophagy. Bioorg. Med. Chem. 34, 116041 (2021)
  22. Yoshimoto S, Katayama K, Suzuki T, Dohmae N & Simizu S. Regulation of N-glycosylation and secretion of Isthmin-1 by its C-mannosylation. Biochim. Biophys. Acta (Gen. Subj.) 1865, 129840 (2021)
  23. Miura K, Suzuki T, Sun H, Takada H, Ishizawa Y, Mizuta H, Dohmae N & Simizu S. Requirement for C-mannosylation to be secreted and activated a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4). Biochim. Biophys. Acta (Gen. Subj.) 1865, 129833 (2021)
  24. Okuyama Y, Kidena M, Kato E, Kawano S, Ishii K, Maie K, Miura K, Simizu S, Sato T & Chida N. Seven-step synthesis of all-nitrogenated sugar derivatives using sequential Overman rearrangements. Angew. Chem. Int. Ed. 60, 5193-5198 (2021)
  25. Osada Y, Suzuki T, Mizuta H, Mori K, Miura K, Dohmae N & Simizu S. The fibrinogen C-terminal domain is seldom C-mannosylated but its C-mannosylation is important for the secretion of microfibril-associated glycoprotein 4. Biochim. Biophys. Acta (Gen. Subj.) 1864, 129637 (2020)
  26. Hayashi S, Osada Y, Miura K & Simizu S. Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1). Biochem. Biophys. Rep. 21, 100734 (2020)
  27. Miura K, Matsuki W, Ogura A, Takao K & Simizu S. Identification of vibsanin A analog as a novel HSP90 inhibitor. Bioorg. Med. Chem. 28, 115253 (2020)
  28. Ishizawa Y, Niwa Y, Suzuki T, Kawahara R, Dohmae N & Simizu S. Identification and characterization of collagen-like glycosylation and hydroxylation of CCN1. Glycobiology 29, 696-704 (2019)
  29. Otake K, Yamada K, Miura K, Sasazawa Y, Miyazaki S, Niwa Y, Ogura A, Takao K & Simizu S. Identification of topoisomerases as molecular targets of cytosporolide C and its analog. Bioorg. Med. Chem. 27, 3334-3338 (2019)
  30. Mizuta H, Kuga K, Suzuki T, Niwa Y, Dohmae N & Simizu S. C-mannosylation of R-spondin2 activates Wnt/β-catenin signaling and migration activity in human tumor cells. Int. J. Oncol. 54, 2127-2138 (2019)
  31. Suto T, Yanagita Y, Nagashima Y, Takikawa S, Kurosu Y, Matsuo N, Miura K, Simizu S, Sato T & Chida N. Unified total synthesis of madangamine alkaloids. Bull. Chem. Soc. Jpn. 92, 545-571 (2019)
  32. Kawahara R, Niwa Y & Simizu S. Integrin β1 is an essential factor in vasculogenic mimicry of human cancer cells. Cancer Sci. 109, 2490-2496 (2018)
  33. Otani K, Niwa Y, Suzuki T, Sato N, Sasazawa Y, Dohmae N & Simizu S. Regulation of granulocyte colony-stimulating factor receptor-mediated granulocytic differentiation by C-mannosylation. Biochem. Biophys. Res. Commun. 498, 466-472 (2018)
  34. Niwa Y, Nakano Y, Suzuki T, Yamagishi M, Otani K, Dohmae N & Simizu S. Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285, 1162-1174 (2018)
  35. Matsuki W, Miyazaki S, Yoshida K, Ogura A, Sasazawa Y, Takao K & Simizu S. Synthesis and evaluation of biological activities of vibsanin A analogs. Bioorg. Med. Chem. Lett. 27, 4536-4539 (2017)
  36. Morishita S, Suzuki T, Niwa Y, Dohmae N & Simizu S. Dpy‑19 like 3‑mediated C‑mannosylation and expression levels of RPE‑spondin in human tumor cell lines. Oncol. Lett. 14, 2537-2544 (2017)
  37. Okamoto S, Murano T, Suzuki T, Uematsu S, Niwa Y, Sasazawa Y, Dohmae N, Bujo H & Simizu S. Regulation of secretion and enzymatic activity of lipoprotein lipase by C-mannosylation. Biochem. Biophys. Res. Commun. 486, 558-563 (2017)
  38. Kunii M, Ohara-Imaizumi M, Takahashi N, Kobayashi M, Kawakami R, Kondoh Y, Shimizu T, Simizu S, Lin B, Nunomura K, Aoyagi K, Ohno M, Ohmuraya M, Sato T, Yoshimura S, Sato K, Harada R, Kim Y-J, Osada H, Nemoto T, Kasai H, Kitamura T, Nagamatsu S & Harada A. Opposing roles for SNAP23 in secretion in exocrine and endocrine pancreatic cells. J. Cell Biol. 215, 121-138 (2016)
  39. Fujiwara M, Kato S, Niwa Y, Suzuki T, Tsuchiya M, Sasazawa Y, Dohmae N & Simizu S. C-mannosylation of R-spondin3 regulates its secretion and activity of Wnt/β-catenin signaling in cells. FEBS Lett. 590, 2639-2649 (2016)
  40. Murahashi M, Simizu S, Morioka M & Umezawa K. Identification of poly(rC) binding protein 2 (PCBP2) as a target protein of immunosuppressive agent 15-deoxyspergualin. Biochem. Biophys. Res. Commun. 476, 445-449 (2016)
  41. Miyazaki S, Sasazawa Y, Mogi T, Suzuki T, Yoshida K, Dohmae N, Takao K & Simizu S. Identification of seco-clavilactone B as a novel small-molecule actin polymerization inhibitor. FEBS Lett. 590, 1163-1173 (2016)
  42. Katsuyama S, Sugino K, Sasazawa Y, Nakano Y, Aono H, Morishita K, Kawatani M, Umezawa K, Osada H & Simizu S. Identification of a novel compound that inhibits osteoclastogenesis by suppressing nucleoside transporters. FEBS Lett. 590, 1152-1162 (2016)
  43. Niwa Y, Suzuki T, Dohmae N & Simizu S. Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells. Mol. Biol. Cell 27, 744-756 (2016)
  44. Tsuchiya M, Niwa Y & Simizu S. N-glycosylation of R-spondin1 at Asn137 negatively regulates its secretion and Wnt/β-catenin signaling-enhancing activity. Oncol. Lett. 11, 3279-3286 (2016)
  45. Sasazawa Y, Sato N, Suzuki T, Dohmae N & Simizu S. C-mannosylation of thrombopoietin receptor (c-Mpl) regulates thrombopoietin-dependent JAK-STAT signaling. Biochem. Biophys. Res. Commun. 468, 262-268 (2015)
  46. Iwasaki A, Ohno O, Katsuyama S, Morita M, Sasazawa Y, Dan S, Simizu S, Yamori T & Suenaga K. Identification of a molecular target of kurahyne, an apoptosis-inducing lipopeptide from marine cyanobacterial assemblages. Bioorg. Med. Chem. Lett. 25, 5295-5298 (2015)
  47. Niwa Y, Suzuki T, Dohmae N & Simizu S. O-fucosylation of CCN1 is required for its secretion. FEBS Lett. 589, 3287–3293 (2015)
  48. Torres-Bacete J, Delgado-Martín C, Gómez-Moreira C, Simizu S & Rodríguez-Fernández JL. The mammalian sterile20-like 1 (Mst1) kinase controls selective CCR7-dependent functions in human dendritic cells. J. Immunol. 195, 973-981 (2015)
  49. Komai K, Niwa Y, Sasazawa Y & Simizu S. Pirin regulates the epithelial to mesenchymal transition independently of Bcl3-Slug signaling. FEBS Lett. 589, 738-743 (2015)
  50. Sasazawa Y, Sato N, Umezawa K & Simizu S. Conophylline protects cells in cellular models of neurodegenerative diseases by inducing mammalian target of rapamycin (mTOR)-independent autophagy. J. Biol. Chem. 290, 6168-6178 (2015)
  51. Noma N, Asagiri M, Takeiri M, Ohmae S, Takemoto K, Iwaisako K, Minato N, Maeda-Yamamoto M, Simizu S & Umezawa K. Inhibition of MMP-2-mediated mast cell invasion by NF-κB inhibitor DHMEQ in mast cells. Int. Arch. Allergy Immunol. 166, 84-90 (2015)
  52. Kuboki M, Ito A, Simizu S & Umezawa K. Activation of apoptosis by caspase-3-dependent specific RelB cleavage in anticancer agent-treated cancer cells: Involvement of positive feedback mechanism. Biochem. Biophys. Res. Commun. 456, 810-814 (2015)
  53. Uematsu S, Goto Y, Suzuki T, Sasazawa Y, Dohmae N & Simizu S. N-glycosylation of Extracellular matrix protein 1 (ECM1) regulates its secretion, which is unrelated to lipoid proteinosis. FEBS Open Bio 4, 879-885 (2014)
  54. Goto Y, Niwa Y, Suzuki T, Uematsu S, Dohmae N & Simizu S. N-glycosylation is required for secretion and enzymatic activity of human hyaluronidase1. FEBS Open Bio 4, 554-559 (2014)
  55. Goto Y, Niwa Y, Suzuki T, Dohmae N, Umezawa K & Simizu S. C-mannosylation of human hyaluronidase 1: Possible roles for secretion and enzymatic activity. Int. J. Oncol. 45, 344-350 (2014)
  56. Matsui C, Ikeda Y, Iinuma H, Kushida N, Kunisada T, Simizu S & Umezawa K. Isolation of a novel paxilline analog pyrapaxilline from fungus that inhibits LPS-induced NO production. J. Antibiot. 67, 787-790 (2014)
  57. Ukaji T, Sasazawa Y, Umezawa K & Simizu S. Involvement of conserved tryptophan residues for secretion of TIMP-2. Oncol. Lett. 7, 631-634 (2014)
  58. Kuroda M, Funasaki S, Saitoh T, Sasazawa Y, Nishiyama S, Umezawa K & Simizu S. Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587, 3656–3660 (2013)
  59. Ogasawara A, Simizu S, Ito A, Kawai T, Saisho Y, Takei I & Umezawa K. Inhibition of NO-induced β-cell death by novel NF-κB inhibitor (-)-DHMEQ via activation of Nrf2-ARE pathway. Biochem. Biophys. Res. Commun. 433, 181-187 (2013)
  60. Arai Y, Iinuma H, Ikeda Y, Igarashi M, Hatano M, Kinoshita N, Ukaji T, Simizu S & Umezawa K. Migracins A and B, new inhibitors of cancer cell migration, produced by Streptomyces sp. J. Antibiot. 66, 225-230 (2013)
  61. Niwa Y, Suzuki T, Dohmae N, Umezawa K & Simizu S. Determination of cathepsin V activity and intracellular trafficking by N-glycosylation. FEBS Lett. 586, 3601-3607 (2012)
  62. Niwa Y, Matsui C, Sukumwang N, Iinuma H, Ikeda Y, Koyano T, Kovitayakorn T, Simizu S & Umezawa K. Inhibition of lysenin-induced hemolysis by all-E-lutein derived from the plant Dalbergia latifolia. Planta Med. 78, 957-961 (2012)
  63. Yasukagawa T, Niwa Y, Simizu S & Umezawa K. Suppression of cellular invasion by glybenclamide through inhibited secretion of platelet-derived growth factor in ovarian clear cell carcinoma ES-2 cells. FEBS Lett. 586, 1504-1509 (2012)
  64. Noma N, Simizu S, Kambayashi Y, Kabe Y, Suematsu M & Umezawa K. Involvement of NF-κB-mediated expression of galectin-3-binding protein in tumor necrosis factor-α-induced brest cancer cell adhesion. Oncol. Rep. 27, 2080-2084 (2012)
  65. Khan MGM, Simizu S, Suzuki T, Masuda A, Muroi M, Kawatani M, Dohmae N & Osada H. Protein disulfide isomerase-mediated disulfide bonds regulate the gelatinolytic activity and secretion of matrix metalloproteinase-9. Exp. Cell Res. 318, 904-914 (2012)
  66. Takeiri M, Horie K, Takahashi D, Watanabe M, Horie R, Simizu S & Umezawa K. Involvement of DNA binding domain in the cellular stability and importin affinity of NF-κB component RelB. Org. Biomol. Chem. 10, 3053-3059 (2012)
  67. Hirai G, Tsuchiya A, Koyama Y, Otani Y, Oonuma K, Dodo K, Simizu S, Osada H & Sodeoka M. Development of a vaccinia H1-related (VHR) phosphatase inhibitor with a nonacidic phosphate-mimicking core structure. ChemMedChem 6, 617-622 (2011)
  68. Khan MGM, Simizu S, Lai NS, Kawatani M, Shimizu T & Osada H. Discovery of a small molecule PDI inhibitor that inhibits reduction of HIV-1 envelope glycoprotein gp120. ACS Chem. Biol. 6, 245-251 (2011)
  69. Miyanishi N, Suzuki Y, Simizu S, Kuwabara Y, Banno K & Umezawa K. Involvement of autocrine CXCL12/CXCR4 system in the regulation of ovarian carcinoma cell invasion. Biochem. Biophys. Res. Commun. 403, 154-159 (2010)
  70. Miyazaki I, Simizu S, Okumura H, Takagi S & Osada H. A small-molecule inhibitor shows that pirin regulates migration of melanoma cells. Nature Chem. Biol. 6, 667-673 (2010)
  71. Kanoh N, Takayama H, Honda K, Moriya T, Teruya T, Simizu S, Osada H & Iwabuchi Y. Cleavable linker for photo-cross-linked small-molecule affinity matrix. Bioconjugate Chem. 21, 182-186 (2010)
  72. Simizu S, Teruya T, Nogawa T, Aono H, Ueki M, Uramoto M, Kobayashi Y & Osada H. Deamino-hydroxy-phoslactomycin B, a biosynthetic precursor of phoslactomycin, induces myeloid differentiation in HL-60 cells. Biochem. Biophys. Res. Commun. 383, 406-410 (2009)
  73. Miyazaki I, Okumura H, Simizu S, Takahashi Y, Kanoh N, Muraoka Y, Nonomura Y & Osada H. Structure-affinity relationship study of bleomycins and Shble protein by use of a chemical array. ChemBioChem 10, 845-852 (2009)
  74. Miyazaki I, Simizu S, Ishida K & Osada H. On-chip fragment-based approach for discovery of high-affinity bivalent inhibitors. ChemBioChem 10, 838-843 (2009)
  75. Takagi S, Simizu S & Osada H. RECK negatively regulates matrix metalloproteinase-9 transcription. Cancer Res. 69, 1502-1508 (2009)
  76. Asami Y, Mori M, Koshino H, Sekiyama Y, Teruya T, Simizu S, Usui T & Osada H. A cell-based screening to detect inhibitors of BRAF signaling pathway. J. Antibiot. 62, 105-107 (2009)
  77. Tamura Y, Simizu S, Muroi M, Takagi S, Kawatani M, Watanabe N & Osada H. Polo-like kinase 1 phosphorylates and regulates Bcl-xL during pironetin-induced apoptosis. Oncogene 28, 107-116 (2009)
  78. Miyazaki I, Simizu S, Ichimiya H, Kawatani M & Osada H. Robust and systematic drug screening method using chemical arrays and the protein library: identification of novel inhibitors of carbonic anhydrase II. Biosci. Biotechnol. Biochem. 72, 2739-2749 (2008) (B.B.B. Excellent Paper Award)
  79. Lai NS, Simizu S, Morisaki D, Muroi M & Osada H. Requirement of the conserved, hydrophobic C-terminus region for the activation of heparanase. Exp. Cell Res. 314, 2834-2845 (2008)
  80. Miyazaki T, Yokoshima S, Simizu S, Osada H, Tokuyama H & Fukuyama T. Synthesis of (+)-vinblastine and its analogues. Org. Lett. 9, 4737-4740 (2007)
  81. Simizu S, Suzuki T, Muroi M, Lai NS, Takagi S, Dohmae N & Osada H. Involvement of disulfide bond formation in the activation of heparanase. Cancer Res. 67, 7841-7849 (2007)
  82. Kanoh N, Asami A, Kawatani M, Honda K, Kumashiro S, Takayama H, Simizu S, Amemiya T, Kondoh Y, Hatakeyama S, Tsuganezawa K, Utata R, Tanaka A, Yokoyama S, Tashiro H & Osada H. Photo-cross-linked small-molecule microarrays as chemical genomic tools for dissecting protein-ligand interactions. Chem. Asian J. 1, 789-797 (2006)
  83. Simizu S, Takagi S, Tamura Y & Osada H. RECK-mediated suppression of tumor cell invasion is regulated by glycosylation in human tumor cell lines. Cancer Res. 65, 7455-7461 (2005)
  84. Kanoh N, Honda K, Simizu S, Muroi M & Osada H. Photo-cross-linked small-molecule affinity matrix for facilitating forward and reverse chemical genetics. Angew. Chem. Int. Ed. 44, 3559-3562 (2005)
  85. Teruya T, Simizu S, Kanoh N & Osada H. Phoslactomycin targets cysteine-269 of the protein phosphatase 2A catalytic subunit in cells. FEBS Lett. 579, 2463-2468 (2005)
  86. Ishida K, Hirai G, Murakami K, Teruya T, Simizu S, Sodeoka M & Osada H. Structure-based design of a selective heparanase inhibitor as an antimetastatic agent. Mol. Cancer Ther. 3, 1069-1077 (2004)
  87. Tamura Y, Simizu S & Osada H. The phosphorylation status and anti-apoptotic activity of Bcl-2 are regulated by ERK and protein phosphatase 2A on the mitochondria. FEBS Lett. 569, 249-255 (2004)
  88. Kondoh M, Suzuki I, Sato M, Nagashima F, Simizu S, Harada M, Fujii M, Osada H, Asakawa Y & Watanabe Y. Kaurene diterpene induces apoptosis in human leukemia cells partly through a caspase-8-dependent pathway. J. Pharmacol. Exp. Ther. 311, 115-122 (2004)
  89. Ishida K, Simizu S, Teruya T, Wierzba MK & Osada H. Rational design and synthesis of novel heparan sulfate mimetic compounds as antiadhesive agents. Bioorg. Med. Chem. Lett. 14, 2505-2509 (2004)
  90. Simizu, S., Tamura, Y. & Osada, H. Dephosphorylation of Bcl-2 by protein phosphatase 2A results in apoptosis resistance. Cancer Sci. 95, 266-270 (2004)
  91. Ishida, K., Teruya, T., Simizu, S. & Osada, H. Exploitation of heparanase inhibitors from microbial metabolites using an efficient visual screening system. J. Antibiot. 57, 136-142 (2004)
  92. Ishida, K., Wierzba, M. K., Teruya, T., Simizu, S. & Osada, H. Novel heparan sulfate mimetic compounds as antitumor agents. Chem. Biol. 11, 367-377 (2004)
  93. Simizu, S., Ishida, K., Wierzba, M. K. & Osada, H. Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines. J. Biol. Chem. 279, 2697-2703 (2004)
  94. Kanoh, N., Kumashiro, S., Simizu, S., Kondoh, Y., Hatakeyama, S., Tashiro, H. & Osada, H. Immobilization of natural products on glass slides by using a photoaffinity reaction and the detection of protein-small-molecule interactions. Angew. Chem. Int. Ed. 42, 5584-5587 (2003)
  95. Nagashima, F., Kondoh, M., Kawase, M., Simizu, S., Osada, H., Fujii, M., Watanabe, Y., Sato, M. & Asakawa, Y. Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata. Planta Med. 69, 377-379 (2003)
  96. Kawatani, M., Uchi, M., Simizu, S., Osada, H. & Imoto, M. Transmembrane domain of Bcl-2 is required for inhibition of ceramide synthesis, but not cytochrome c release in the pathway of inostamycin-induced apoptosis. Exp. Cell Res. 286, 57-66 (2003)
  97. Simizu, S., Ishida, K., Wierzba, M. K., Sato, T.-A. & Osada, H. Expression of heparanase in human tumor cell lines and human head and neck tumors. Cancer Lett. 193, 83-89 (2003)
  98. Matsumoto, Y., Kawatani, M., Simizu, S., Tanaka, T., Takada, M. & Imoto, M. Bcl-2-independent induction of apoptosis by neuropeptide receptor antagonist in human small cell lung carcinoma cells. Anticancer Res. 20, 3123-3129 (2000)
  99. Kawatani, M., Simizu, S., Osada, H., Takada, M., Arber, N. & Imoto, M. Involvement of protein kinase C-regulated ceramide generation in inostamycin-induced apoptosis. Exp. Cell Res. 259, 389-397 (2000)
  100. Simizu, S. & Osada, H. Mutations in the Plk gene lead to instability of Plk protein in human tumour cell lines. Nature Cell Biol. 2, 852-854 (2000)
  101. Simizu, S., Shibasaki, F. & Osada, H. Bcl-2 inhibits calcineurin-mediated Fas ligand expression in antitumor drug-treated baby hamster kidney cells. Jpn. J. Cancer Res. 91, 706-714 (2000)
  102. Simizu, S., Takada, M., Umezawa, K. & Imoto, M. Requirement of caspase-3(-like) protease-mediated hydrogen peroxide production for apoptosis induced by various anticancer drugs. J. Biol. Chem. 273, 26900-26907 (1998)
  103. Simizu, S., Tanabe, K., Tashiro, E., Takada, M., Umezawa, K. & Imoto, M. Potentiation of paclitaxel cytotoxicity by inostamycin in human small cell lung carcinoma, Ms-1 cells. Jpn. J. Cancer Res. 89, 970-976 (1998)
  104. Tashiro, E., Simizu, S., Takada, M., Umezawa, K. & Imoto, M. Caspase-3 activation is not responsible for vinblastine-induced Bcl-2 phosphorylation and G2/M arrest in human small cell lung carcinoma Ms-1 cells. Jpn. J. Cancer Res. 89, 940-946 (1998)
  105. Imoto, M., Tanabe, K., Simizu, S., Tashiro, E., Takada, M. & Umezawa, K. Inhibition of cyclin D1 expression and induction of apoptosis by inostamycin in small cell lung carcinoma cells. Jpn. J. Cancer Res. 89, 315-322 (1998)
  106. Simizu, S., Umezawa, K., Takada, M., Arber, N. & Imoto, M. Induction of hydrogen peroxide production and Bax expression by caspase-3(-like) proteases in tyrosine kinase inhibitor-induced apoptosis in human small cell lung carcinoma cells. Exp. Cell Res. 238, 197-203 (1998)
  107. Simizu, S., Imoto, M., Masuda, N., Takada, M. & Umezawa, K. Involvement of hydrogen peroxide production in erbstatin-induced apoptosis in human small cell lung carcinoma cells. Cancer Res. 56, 4978-4982 (1996)
  108. Simizu, S., Imoto, M. & Umezawa, K. Induction of apoptosis by erbstatin in mouse leukemia L1210 cells. Biosci. Biotechnol. Biochem. 58, 1549-1552 (1994)

Review Articles

  1. Simizu S. Vasculogenic mimicry: A dynamic event of malignancys. Biochim. Biophys. Acta (gen. Subj) 1866, 130084 (2022)(Editorial)
  2. Takao K, Ogura A, Yoshida K, & Simizu S. Total synthesis of natural products using intramolecular Nozaki-Hiyama-Takai-Kishi reactions. Synlett 31, 421-433 (2020)
  3. Niwa Y, & Simizu S. C-mannosylation: previous studies and future research perspectives. Trends Glycosci. Glycotechnol. 30, E231-E238 (2018)
  4. Umezawa K, Kojima I, Simizu S, Lin Y, Fukatsu H, Koide N, Nakade Y & Yoneda M. Therapeutic activity of plant-derived alkaloid conophylline on metabolic syndrome and neurodegerative disease models. Human Cell 31, 95-101 (2018)
  5. Simizu S & Niwa Y. Molecular targets for suppression of metastasis: recent cellular observations. For. Immunopathol. Dis. Ther. 4, 43-51 (2013)
  6. Khan MGM, Simizu S, Kawatani M & Osada H. The potential of protein disulfide isomerase as a therapeutic drug target. Oncol. Res. 19, 445-453 (2012)
  7. Miyazaki I, Simizu S & Osada H. The application of the chemical array for biological study. Methods Mol. Biol. 669, 95-107 (2010)
  8. Simizu S, Ishida K & Osada H. Heparanase as a molecular target of cancer chemotherapy. Cancer Sci. 95, 553-558 (2004)

Review Articles in Japanese

  1. 丹羽祐貴、清水史郎「C-マンノシル化のこれまでの研究動向と今後の研究展望」Trends Glycosci. Glycotechnol.,30, J191-J198 (2018)
  2. 叶直樹、清水史郎「化合物アレイを起点としたケミカルバイオロジー 化合物アレイの基盤技術開発から機能未知タンパク質の機能解析まで」化学と工業, 64, 37-39 (2011)
  3. 宮崎功、奥村英夫、清水史郎、長田裕之「小分子化合物TPh Aを用いた初のピリン機能解明」実験医学, 29, 79-83 (2011)
  4. 清水史郎、辻田和彦、長田裕之「分子細胞学的解析」日本臨牀, 67増刊号1, 143-147 (2009)
  5. 清水史郎、長田裕之「バイオプローブ開発からケミカルバイオロジー研究へ」化学工業, 58, 335-339 (2007)
  6. 叶直樹、清水史郎、臼井健郎、長田裕之「天然由来のバイオプローブを用いたケミカルジェネティクスとケミカルゲノミクス」蛋白質 核酸 酵素, 50, 1037-1042 (2005)
  7. 臼井健郎、叶直樹、清水史郎、長田裕之「バイオプローブの探索と細胞内標的分子の同定」実験医学, 23, 1803-1809 (2005)
  8. 清水史郎「2004年度日本癌学会奨励賞―薬剤耐性･転移に関与するがん分子標的の機能解析」Medical Academy NEWS, 929, 6 (2005)
  9. 清水史郎、長田裕之「微小管作用薬の抗がん作用」分子細胞治療, 2, 364-369 (2001)
  10. 長田裕之、浜本隆二、清水史郎「進化する抗がん剤―第４回がん分子標的治療研究会見聞録」化学と生物, 38, 799-803 (2000)
  11. 清水史郎「抗腫瘍物質による細胞死の誘導機構」バイオサイエンスとインダストリー, 56, 747-750 (1998)
  12. 井本正哉、清水史郎、梅沢一夫「チロシンキナーゼ阻害剤のアポトーシス誘導機構」日本農芸化学会誌, 71, 523-526 (1997)

Books

  1. 「生命の経済」慶應義塾大学出版会、西尾宇広（編）(2020)
  2. 「がん分子標的治療研究ハンドブック」金芳堂、曽根三郎／鶴尾隆（編）(2009)
  3. 「マイクロアレイ・バイオチップの最新技術」シーエムシー出版、伊藤嘉浩（監）(2007)
  4. 「がんの分子標的治療」南山堂、鶴尾隆（編）(2008)
  5. 「細胞生物学事典」朝倉書店、石川統・黒岩常祥・永田和宏（編）(2005)
  6. “Bioprobes-Biochemical tools for investigating cell function” Springer, Osada, H. (Ed.), (2000)

Prizes

  1. 日本がん転移学会　研究奨励賞 (2011)
  2. 日本農芸化学会　B.B.B.論文賞 (2009)
  3. 日本癌学会　奨励賞 (2004)
  4. 慶應義塾大学　理工学部　藤原賞 (1998)

Grants (Representative and External only)

  1. 公益財団法人天野工業技術研究所「血管擬態を標的とした抗がん剤開発」（令和5-6年度）
  2. 公益財団法人テルモ生命科学振興財団 2022年度研究開発助成「AXLタンパク質におけるC-mannosylationの役割解析とその制御」 （令和5年度）
  3. 公益財団法人水谷糖質科学振興財団 第27回研究助成「ヒト新規C型糖修飾酵素の同定と機能解析」 (令和2年度)
  4. 科学研究費・基盤研究(C)「新たなC型糖修飾責任酵素の同定と基質タンパク質の解析」 (平成30-32年度)
  5. 公益財団法人コスメトロジー研究振興財団「メラニン生成関連タンパク質における新規糖修飾の解析と応用への展開」 (平成29-30年度)
  6. 公益財団法人天野工業技術研究所「新規C型糖修飾酵素阻害剤の開発」 (平成29年度)
  7. 科学研究費・基盤研究(B)「Pirin阻害剤による骨疾患薬の開発」 (平成24-26年度)
  8. 科学研究費・新学術領域（計画）「ケミカルバイオロジーによる細胞内物流システムの理解と制御」 (平成20-24年度)
  9. 科学研究費・若手研究(A)「糖鎖修飾によるがん転移制御機構の化学生物学的解析」 (平成18-20年度)
  10. 科学研究費・若手研究(B)「糖鎖修飾によるタンパク質活性化調節機構の解析」 (平成16, 17年度)
  11. 科学研究費・特定領域研究(C)「ヘパラナーゼ阻害剤探索系の構築と活性化機構の解析」 (平成13年度)
  12. 科学研究費・基盤研究(C)「微小管傷害で活性化されるBcl-2リン酸化酵素の同定と機能解析」 (平成13, 14年度)
  13. 科学研究費・特定領域研究(C)「ヘパラナーゼ阻害剤探索系の構築と活性化機構の解析」 (平成12年度)

Lectures Invited

  1. Simizu S.「Identification of novel C-mannosylated proteins and its function」2023 Annual Meeting Society for Glycobiology、Hilton Waikoloa Village Resort Big Island、USA、2023.11.8
  2. 清水史郎「がん悪性化因子の解析～血管擬態とC型糖修飾を中心として～」、公益財団法人微生物化学研究会、Fridayセミナー、オンライン、2021.06.11
  3. 清水史郎「ケミカルバイオロジー研究による血管擬態解明」、日本ケミカルバイオロジー学会・産学連携委員会「ケミカルバイオロジーの技術革新」、研究会、オンライン、2021.03.16
  4. 清水史郎「C-mannosylation: 酵素と基質の探索」、山口大学大学院医学系研究科 薬理学講座、ラボセミナー、山口大学、2021.03.12
  5. Simizu S.「C-mannosylation: identification of novel enzymes and substrates」The 7th RIKEN-Max Planck Institute Joint Symposium、Schloss-Ringberg、Max Planck、Munich、2019.9.16
  6. 清水史郎「低分子化合物による細胞機能の解析」ケミカルバイオロジー、長浜バイオ大学、2019.5.8
  7. 清水史郎「C-mannosylation–基質と酵素の探索」、日本農芸化学会中部支部 第183回支部例会、ミニシンポジウム、名古屋大学、2018.9.15
  8. 清水史郎「非典型糖修飾C-mannosylationの役割と責任酵素の解析」、2017年度生命科学系学会合同年次大会 (ConBio2017)、ワークショップ、神戸商工会議所、2017.12.06
  9. 清水史郎「C-mannosylationの機能解析と責任酵素の同定」、理研シンポジウム、理化学研究所、2017.1.20
  10. 清水史郎「ヒトCCN1の糖鎖修飾解析と分泌における役割」、第8回日本CCNファミリー 研究会、岡山大学、2016.8.27
  11. Simizu S.「Identification of a compound that regulates autophagy in cells」 International Mini Symposium on Chemical Biology of Natural Products、Keio University、Yokohama、2015.3.24
  12. Simizu S.「Regulation of Wnt signaling by glycosylation」Invited lecture、Universiti Sains Malaysia、Malaysia、2014.3.17
  13. 清水史郎「細胞内ロジスティクス研究におけるケミカルバイオロジーの役割」 新学術領域研究「ロジスティクス」終了「オートファジーの集学的研究：分子基盤か ら疾患まで」発足合同シンポジウム、淡路夢舞台 (淡路島)、2013.9.18
  14. Simizu S, Goto Y & Niwa Y.「Involvement of glycosylation in hyaluronidase1 functions」9th International Conference on Hyaluronan、Renaissance Oklahoma City Convention Center Hotel、USA、2013.6.4
  15. 清水史郎「がん分子標的研究の今昔」、理研セミナー、理化学研究所、2012.7.13
  16. Simizu S & Osada H.「Regulation of melanoma cell migration by Pirin revealed by an approach of chemical biology」第70回日本癌学会学術総会、International Session、名古屋国際会議場、2011.10.5
  17. 清水史郎、長田裕之「ケミカルバイオロジーと細胞内ロジスティクス研究の融合」、日本生化学会、シンポジウム、京都国際会館、2011.9.21
  18. 清水史郎「化合物アレイを用いたタンパク質阻害剤の網羅的探索」、日本薬学会第131年会、一般シンポジウム、ツインメッセ静岡、2011.3.29
  19. 清水史郎「化合物アレイを用いた新規転移抑制剤の探索」癌研セミナー、財団法人癌研究会吉田富三記念講堂、2010.2.16
  20. 清水史郎「化合物アレイを用いた低分子阻害剤探索系の構築」合同酒精株式会社セミナー、合同酒精株式会社酵素医薬品研究所、2009.7.31
  21. 清水史郎、宮崎功、長田裕之「最先端創薬システム：分子標的治療薬開発のためのケミカルバイオロジー」第13回日本がん分子標的治療学会学術集会、シンポジウム、ホテルクレメント徳島、2009.6.25
  22. Simizu S, Miyazaki I & Osada H.「Establishment of novel antitumor drugs screening method by using chemical array」AACR-ACS Joint Conference on Chemistry in Cancer Research、Westin New Orleans Canal Place、USA、2009.2.9
  23. 清水史郎、長田裕之「化合物アレイを用いた新たな低分子阻害剤探索系の構築」特定領域研究「トラフィック」終了・新学術領域研究「ロジスティクス」発足合同シンポジウム、航空会館 (東京)、2009.1.29
  24. Simizu S.「Human heparanase: Activation and inhibition」第15回PG Forum、東京医科歯科大学、2007.10.28
  25. Simizu S, Miyazaki I, Okumura H, Kanoh N & Osada H.「A novel chemical microarray platform for searching anticancer drugs」第66回日本癌学会学術総会、International Session、パシフィコ横浜、2007.10.3
  26. 清水史郎「癌の転移・浸潤機構解析とその阻害剤開発」バイオトピックス、長浜バイオ大学、2006.10.19
  27. 清水史郎「アポトーシス誘導におけるHsp90の役割」理研シンポジウム、第3回マルチバイオプローブ研究の新展開、長井記念館、2001.2.9

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